Study of the radiomodifier effect of Pityrocarpa moniliformis extract
DOI:
https://doi.org/10.15392/bjrs.v7i2A.760Keywords:
Biomphalaria glabrata, Pityrocarpa moniliformis, RadiationAbstract
Ionizing radiation has been applied in several areas of knowledge, among them the study of the radiomodifier activity of natural substances. These substances can modify the cellular response to the damage induced by the radiation. Therefore, this work aimed to evaluate the radiomodifier action of Pityrocarpa moniliformis extract on Biomphalaria glabrata embryos exposed to 60Co gamma radiation. Initially, toxicity tests were performed on the extract against the B. glabrata embryos for the choice of concentration that did not cause death and embryonic malformation. Then, the antioxidant activity of the P. moniliformis extract with flavonoids and phenolic compounds was evaluated by means of the ABTS method. To evaluate the radiomodifier activity of the extract, embryos were selected in the blastula stage and irradiated with 7.5 Gy in a 60Co source (gammacell-Co60). Then, the embryos were exposed for 24 h to the extract of P. moniliformis at a concentration of 250 μg/mL. The results showed that the extract of P. moniliformis presents flavonoids and enzymatic inhibition by ABTS, which demonstrates the presence of antioxidant compounds. However, the tests of the radiomodifier activity did not present radioprotective effect for embryos exposed to ionizing radiation.Downloads
References
KAPLAN, I. Física Nuclear, 1st ed. Rio de Janeiro: Guanabara Dois, 1978.
BITELLI, T. Física e dosimetria das radiações, 2nd ed. São Paulo: Atheneu, 2006.
BOLUS, N. E. Basic review of radiation biology and terminology. JNMT, v. 29, p. 67-73, 2001.
MULLER, H. J. Artificial Transmutation of the Gene. Science, v. 66, p. 84-87, 1927.
HALL, E. J. Radiobiology for the Radiologist, 6th ed. Philadelphia: Lippincott Wilkins & Williams, 2006.
ANNO, G. H., YOUNGBLOOM, R. W. R. M., MERCIER, J. R. Dose response relationships for acute ionizing radiation lethality. Health Phys, v. 84, p. 568-575, 2003.
ARORA, R. Botanicals as potential radioprotective and radiorecovery agents: Current status and emerging directions for clinical applications, In: PROCEEDINGS OF THE WORLD AYURVEDA CONFERENCE (in press), 2007, Pune, November, 2007, p. 5-12.
JAGETIA, G. C. Radioprotective potential of plants and herbs against the effects of ionizing radiation. J Clin Biochem Nutr, v. 40, p. 74, 2007.
OLIVEIRA-FILHO, E. C. Efeitos de substâncias químicas sobre a reprodução de moluscos de água doce: estudos com caramujos do gênero Biomphalaria. 2003. Tese (Doutorado em Saúde Pública) - Escola Nacional de Saúde Pública, Fundação Oswaldo Cruz, Rio de Janeiro, p. 138.
PHILLIPSON, J. D., ANDERSON, L. A. Ethnopharmacology and Western Medicine. J Ethnopharmacol, v. 25, p. 61-72, 1989.
LEE, T. K., O’BRIEN, K. F., WANG, W., JOHNKE, R. M., SHENG, C., BENHABIB, S. M., WANG, T., ALLISON, R. R. Radioprotective effect of American ginseng on human lymphocytes at 90 minutes postirradiation: a study of 40 cases. J Altern Complement Med, v. 16, p. 561-567, 2010.
RE, R., PELEGRINI, N., PROTEGGENTE, A., PANNALA, A., YANG, M., RICE-EVANS, C. Antioxidant activity applying and improved ABTS radical cátion decolorization assay. Free Radic Biol Med, v. 26, p.1231-1237, 1999.
LI, H. B., WONG, C. C., CHENG, K. W., CHEN, F. Antioxidant properties in vitro and total phenolic contents in methanol extracts from medicinal plants. Food Sci Technol, v. 41, p. 385-390, 2008.
WOISKY, R. G., SALATINO, A. Analysis of propolis: some parameters and procedures for chemical quality control. J Apic Res, v. 37, p. 99-105, 1998.
SILVA, M. L. C., COSTA, R. S., SANTANA, A. S., KOBLITZ, M. G. B. Phenolic compounds, carotenoids and antioxidant activity in plant products. Semina: Ciênc Agrár, v. 31, p. 669-682, 2010.
ROCHA-FILHO, C. A., ALBUQUERQUE, L. P., SILVA, L. R., SILVA, P. C., COELHO, L. C., NAVARRO, D. M., ALBUQUERQUE, M. C., MELO, A.M.M.A., NAPOLEÃO, T. H., PONTUAL, E. V., PAIVA, P. M. Assessment of toxicity of Moringa oleifera flower extract to Biomphalaria glabrata, Schistosoma mansoni and Artemia salina. Chemosphere, v. 132, p. 188-192, 2015.
OKAZAKI, K., ANDRADE JÚNIOR, H. F., KAWANO, T. Effect of 60Co gamma radiation on Biomphalaria glabrata (Mollusca, Gastropoda) embryos: mortality, malformation and hatching. Braz J Med Biol Res, v. 29, p. 1057-1067, 1996.
SIQUEIRA, W. N., SILVA, L. R. S., SILVA, R. C., LACERDA, L. B. N., SILVA, H. A. M. F., SANTOS, M. L. O., SÁ, J. L. F., SILVA, E. B., MELO, A. M. M. A. Radioprotective effect of the extract of Ziziphus joazeiro and Anacardium occidentale on embryos of Biomphalaria glabrata submitted to ionizing radiation, In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, 2011, Belo Horizonte: Comissão Nacional de Energia Nuclear, 2011. p. 1-10.
Published
Issue
Section
Categories
License
Copyright (c) 2021 Brazilian Journal of Radiation Sciences
This work is licensed under a Creative Commons Attribution 4.0 International License.
Licensing: The BJRS articles are licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
How to Cite
