The influence of generator eluate in radiolabeling PSMA-11 kit with 68Ga

Autores/as

  • Bianca Franchesqueti Vivaldini Instituto de Pesquisas Energéticas e Nucleares image/svg+xml
  • Elaine Bortoleti de Araújo Instituto de Pesquisas Energéticas e Nucleares image/svg+xml

DOI:

https://doi.org/10.15392/bjrs.v9i1A.1541

Palabras clave:

prostate cancer, radiolabeling, radiopharmacy

Resumen

Gaining prominence in clinical practice, the 68Ga, positron emitter radionuclide easily obtained by 68Ge/68Ga generator elution, has shown potential and excellent quality on radiolabeling of peptide for use in positron emission tomography (PET), in particular urea-based inhibitor peptides, directed to the prostate-specific membrane receptor (PSMA). Previous studies with the PSMA linked to the chelator HBED-CC (PSMA-11) radiolabeled with 68Ga showed high contrast PET/CT images to evaluate recurrence and metastasis of prostate cancer, becoming an important imaging agent in the clinical routine. This work intended to evaluate the influence of the quality of the 68Ge/68Ga generator eluate in direct labelling of PSMA-11 with 68Ga, assisting in the development of kit for prompt radiolabeling. It was evaluated the 68GaCl3 eluate from 68Ge/68Ga non-GMP generator (manufacturer A) and 68Ge/68Ga GMP generator (manufacturer B), both commercially available. To evaluate the influence of  the 68Ga eluate on radiochemical yield of the preparations,  the radiochemical purity was determined by thin layer chromatography and HPLC. The radiolabeling with non-GMP generator eluate was made with and without preliminary purification of the 68 gallium chloride eluate, employing cationic purification columns. The results showed higher radiochemical yield with the 68GaCl3 eluate from the 68Ge/68Ga GMP generator, obtaining the radiolabeled product more easily and speed to clinical practice, without preliminary purification, as opposed to the use of non-GMP 68Ge/68Ga generator which required preliminary purification of the 68GaCl3 eluate to promote satisfactory radiochemical purity results.

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Biografía del autor/a

  • Bianca Franchesqueti Vivaldini, Instituto de Pesquisas Energéticas e Nucleares
    Centro de Radiofarmácia
  • Elaine Bortoleti de Araújo, Instituto de Pesquisas Energéticas e Nucleares
    Centro de Radiofarmácia

Referencias

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PACKER, J.; MAITLAND, N. The molecular and cellular origin of human prostate cancer. Biochimica et Biophysica Acta 1863, p. 1238-1260, 2016.

AFSHAR-OROMIEH, A. et al. Comparison of PET imaging with a 68Ga-labelled PSMA ligand and 18F-choline-based PET/CT for the diagnosis of recurrent prostate cancer. Eur J Nucl Med Mol Imaging, v. 42, p. 197–209, 2015.

MIYAHIRA, A.; et al. Meeting report from the Prostate Cancer Foundation PSMA-directed radionuclide scientific working group. The Prostate, 2018, p. 1-15, 2018.

VELIKYAN, I. Prospective of 68Ga-Radiopharmaceutical Development. Theranostics, Volume 4, p.47–80, 2013.

BANERJEE, S. R.; POMPER, M. G. Clinical applications of Gallium-68. Appl Radiat Isot., v. 76, p. 2–13, 2013.

Gallium (68Ga) PSMA 11 injection, European pharmacopoeia, 2018.

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Publicado

2021-04-30

Número

Vol. 9 Núm. 1A (2021)

Sección

The Meeting on Nuclear Applications (ENAN) 2019

Licencia

Derechos de autor 2021 Brazilian Journal of Radiation Sciences (BJRS)

Creative Commons License

Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.

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Cómo citar

The influence of generator eluate in radiolabeling PSMA-11 kit with 68Ga. Brazilian Journal of Radiation Sciences (BJRS), Rio de Janeiro, Brazil, v. 9, n. 1A, 2021. DOI: 10.15392/bjrs.v9i1A.1541. Disponível em: https://www.bjrs.org.br/revista/index.php/REVISTA/article/view/1541. Acesso em: 21 jul. 2025.